CRISPR Screen Dataset
Alborzinia H (2023) - 1-PMID37435859
Title: LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastoma.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Neuroblastoma Cell Line [BTO:0000932, EFO:0005214]
- Cell Line : SK-N-DZ [BTO:0004217, CELLOSAURUS:CVCL_1701]
- Phenotype : Response To Chemicals
- Condition : 1S,3R-RSL3 (300.0 nM) [PubChem]
- Library : Human CRISPR Activation Pooled Library (Calabrese) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: dCas9-VP64 & p65-HSF1 (CRISPR SAM) | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 949 | Full Dataset Size: 18,883
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: < 0.3
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR-activation (CRISPRa) screen was performed to identify novel regulators of ferroptosis. This CRISPRa_RSL3 screen involved positive selection for viability after exposing the cells to the ferroptosis inducing agent RSL-3 which inhibits GPX4.
Score Distribution
< 8.394513033
> -7.095569452