CRISPR Screen Dataset
Kawabata A (2022) - 3-PMID35723366
Title: CRISPR/Cas9 Screening for Identification of Genes Required for the Growth of Ovarian Clear Cell Carcinoma Cells.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Ovarian Cancer Cell Line [BTO:0000811, EFO:0002394]
- Cell Line : ES-2 [BTO:0004424, CELLOSAURUS:CVCL_3509]
- Phenotype : Cell Proliferation
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAGeCK-MLE | Number of Hits: 4,610 | Full Dataset Size: 18,053
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Beta Score) <= -0.6 AND Score.2 (p-Value) <= 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in decreased growth of the ovarian clear cell carcinoma ES-2 cell line
Score Distribution
< 0.74647
> -5.6496