CRISPR Screen Dataset
McLellan LK (2021) - 1-PMID33513212
Title: A host receptor enables type 1 pilus-mediated pathogenesis of Escherichia coli pyelonephritis.
Screen Details:
- Screen Rationale : Decreased binding of bacteria
- Cell Type : Renal Medulla Cell Line [BTO:0001526]
- Cell Line : IMCD-3 [BTO:0003913]
- Phenotype : Response To Bacteria
- Condition : Escherichia coli (str. UTI89) (150.0 MOI) [NCBI Taxid 364106]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 9 | Full Dataset Size: 20,144
- Experimental Setup : Bacteria Exposure | Duration: 75 Minutes | MOI: = 0.5
- Significance : Authors have indicated that results with Score.1 (p-Value) > 2.58 AND Score.2 (Log2FC) > 0.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in decreased binding of uropathogenic Escherichia coli to kidney cells in vitro
Score Distribution
< 6.093119218
> 0.327247148