CRISPR Screen Dataset
Matía A (2022) - 27-PMID36574441
Title: Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Response To Virus
- Condition : Virus:Vaccinia virus Western Reserve (0.3 MOI) [NCBI Taxid 696871]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 10 | Full Dataset Size: 20,699
- Experimental Setup : Virus Exposure | Duration: 2 Weeks
- Significance : Authors have indicated that results with Score.1 (FDR) < 0.05 AND Score.2 (Log2FC) > 0.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased resistance to Vaccinia Virus infection
- In an initial screen authors found no surviving cells and surmised that this may be due to subsequent rounds of infection due to the presence of progeny virus. To address this they used viruses deficient in the production of progeny due to a deficiency in the D4R gene (V-D4)and varying MOI in rounds of infection (1st round=0.3, 2nd round=0.6, 3rd round=0.8, 4th round=1)
Score Distribution
< 0.999999
> 0.00099