CRISPR Screen Dataset
Trimarco JD (2022) - 1-PMID36309510
Title: Cellular glycan modification by B3GAT1 broadly restricts influenza virus infection.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A-549 [BTO:0000018, CELLOSAURUS:CVCL_0023]
- Phenotype : Response To Virus
- Condition : Virus: Influenza B virus (B/Yamagata/16/1988) (5 MOI) [NCBI Taxid 416674]
- Library : Human CRISPR Activation Pooled Library (Calabrese) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: dCas9-VP64 | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 6 | Full Dataset Size: 18,885
- Experimental Setup : Virus Exposure | Duration: 9 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 0.00024 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Activation of the hit genes results in increased resistance to Influenza B
Score Distribution
< 0.99998
> 0.000000199