CRISPR Screen Dataset
Wu L (2022) - 1-PMID35981571
Title: Genome-wide CRISPR screen identifies MTA3 as an inducer of gemcitabine resistance in pancreatic ductal adenocarcinoma.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Pancreatic Adenocarcinoma Cell Line [BTO:0001521]
- Cell Line : BxPC-3 [BTO:0001861, CELLOSAURUS:CVCL_0186]
- Phenotype : Response To Chemicals
- Condition : Gemcitabine (2.0 nM) [ChEBI]
- Library : Human SAM library in lentiSAMv2 backbone [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 618 | Full Dataset Size: 18,947
- Experimental Setup : Drug Exposure | Duration: 3 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 AND Score.3 (Log2FC) >= 2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Activation of the hit genes results in increased resistance to gemcitabine in pancreatic ductal adenocarcinoma
Score Distribution
< 0.99998
> 0.00000703