CRISPR Screen Dataset

Israeli M (2022) - 5-PMID35469023

H. sapiens

Title: Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2.

Screen Rationale : Cell-essential genes
Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
Phenotype : Viability [APO:0000111]
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 1,989 | Full Dataset Size: 19,115
Experimental Setup : Timecourse | Duration: 7 Days | MOI: ~ 0.3
Significance : Authors have indicated that results with Score.1 (Log2FC) < 0.0 AND Score.2 (p-Value) < 0.05 OR Score.1 (Log2FC) > 0.0 AND Score.3 (p-Value) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A genome-wide CRISPR/Cas9 screen was performed in Calu-3 cells to identify cell-essential genes affecting cell growth or viability in the absence of any SARS-CoV-2 virus.

< 1.6965

> -5.655