CRISPR Screen Dataset
Israeli M (2022) - 4-PMID35469023
Title: Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
- Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (B.1.351 Beta variant) (0.02 MOI)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 17 | Full Dataset Size: 19,114
- Experimental Setup : Virus Exposure | Duration: 9 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR knockout screen was carried out in human Calu-3 cells exposed to the SARS-CoV-2 coronavirus to identify enriched proviral gene hits (positive scores) and depleted antiviral gene hits (negative scores). This screen identifies host factors important for the SARS-CoV-2 B.1.135 Beta variant.
Score Distribution
< 1.8333
> -4.9684