CRISPR Screen Dataset
Chagraoui J (2021) - 3-PMID33417871
Title: UM171 Preserves Epigenetic Marks that Are Reduced in Ex Vivo Culture of Human HSCs via Potentiation of the CLR3-KBTBD4 Complex.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : OCI-AML-5 [BTO:0004621, CELLOSAURUS:CVCL_1620]
- Phenotype : Response To Chemicals
- Condition : UM171 (800 nM) [PubChem]
- Library : Extended Knockout Library (EKO) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAGeCK-MLE | Number of Hits: 33 | Full Dataset Size: 18,923
- Experimental Setup : Drug Exposure | Duration: 8 Days
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a positive beta score show increased resistance to UM171 upon KO
- Hit genes with a negative beta score show increased sensitivity to UM171 upon KO
Score Distribution
< 3.2023
> -2.6875