CRISPR Screen Dataset
Wei LH (2023) - 2-CUSTOM00000022
Title: Genome-wide CRISPR screens identify noncanonical translation factor eIF2A as an enhancer of SARS-CoV-2 programmed –1 ribosomal frameshifting
Screen Details:
- Screen Rationale : Increased/decreased viral programmed -1 ribosomal frameshifting (-1 PRF)
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Regulation Of Viral Programmed -1 Ribosomal Frameshifting (-1 PRF)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 442 | Full Dataset Size: 21,926
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: < 0.3
- Significance : Authors have indicated that results with Score.1 (Log2FC) < 0.0 AND Score.2 (p-Value) < 0.01 OR Score.1 (Log2FC) > 0.0 AND Score.3 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A reporter-based genome-wide CRISPR/Cas9 knockout screen was carried out to identify human host modulators of SARS-CoV-2 programmed -1 ribosomal frameshifting (-1 PRF), a process used by coronaviruses to regulate the translation of their polycistronic viral RNAs. A dual fluorescent protein-based reporter was used in this knockout screen to quantify SARS-CoV-2 -1 PRF efficiency. Gene hits with negative log fold changes (LFCs) are -1 PRF suppressors and those with positive LFCs are enhancers. (14-day timecourse)
Score Distribution
< 1.2768
> -0.868