CRISPR Screen Dataset
Thandapani P (2022) - 1-PMID34937946
Title: Valine tRNA levels and availability regulate complex I assembly in leukaemia.
Screen Details:
- Screen Rationale : Response to valine restriction
- Cell Type : Acute Lymphoblastic Leukemia Cell Line [BTO:0002144]
- Cell Line : Unknown
- Phenotype : Cell Proliferation
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CERES | Number of Hits: 26 | Full Dataset Size: 18,414
- Experimental Setup : Other | Duration: 14 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a negative CERES score show decreased growth when valine is restricted (sensitizer genes). Genes with a positive CERES score show increased growth when valine is restricted (resistor genes)
Score Distribution
< 2.109294755
> -4.109278626