CRISPR Screen Dataset

Israeli M (2022) - 1-PMID35469023

H. sapiens

Title: Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2.

Screen Rationale : Increased/decreased resistance to virus
Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
Phenotype : Response To Virus
Condition : Virus: SARS-CoV-2 (isolate USA-WA1/2020) (0.02 MOI)
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 26 | Full Dataset Size: 19,114
Experimental Setup : Virus Exposure | Duration: 9 Days | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.0 AND Score.2 (FDR) < 0.45 OR Score.1 (Log2FC) < 0.0 AND Score.3 (FDR) < 0.075 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A genome-wide CRISPR knockout screen was carried out in human Calu-3 cells exposed to the SARS-CoV-2 coronavirus to identify enriched proviral gene hits (positive scores) and depleted antiviral gene hits (negative scores). This screen identifies host factors important for the wild type SARS-CoV-2 coronavirus as well as the alpha and beta variants (VOCs).

< 1.5346

> -4.4178