CRISPR Screen Dataset

Nguyen NH (2022) - 3-PMID36111891

H. sapiens

Title: Genome-wide CRISPR/Cas9 Screen Identifies Etoposide Response Modulators Associated with Outcomes in Pediatric AML.

Screen Rationale : Increased/decreased drug resistance
Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
Phenotype : Response To Chemicals
Condition : Etoposide (0.1 µM) [ChEBI]
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 91 | Full Dataset Size: 19,114
Experimental Setup : Drug Exposure | Duration: 18 Days | MOI: ~ 0.3
Significance : Authors have indicated that ALL results are to be considered significant.

Genes with a positive score are those whose KO results in increased resistance to etoposide, so the gene normally functions as an etoposide sensitivity gene
Genes with a negative score are those whose KO results in increased sensitivity to etoposide, so the gene normally functions as an etoposide resistance gene
Hit genes were those with an FDR<0.1

< 10.027

> -7.579