CRISPR Screen Dataset
Sapp V (2021) - 1-PMID34230586
Title: Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Cardiac Muscle Cell Line [BTO:0003265]
- Cell Line : cardiomyocyte cell line [BTO:0003324]
- Phenotype : Response To Chemicals
- Condition : Doxorubicin (3.0 µM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RIGER | Number of Hits: 169 | Full Dataset Size: 16,095
- Experimental Setup : Drug Exposure | Duration: 72 Hours | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased resistance to doxorubicin toxicity in iPS derived cardiomyocytes
Score Distribution
< 3.93
> -5.69