CRISPR Screen Dataset
Cai W (2022) - 1-PMID35657206
Title: A Genome-Wide Screen Identifies PDPK1 as a Target to Enhance the Efficacy of MEK1/2 Inhibitors in NRAS Mutant Melanoma.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : WM1361A [CELLOSAURUS:CVCL_6788]
- Phenotype : Response To Chemicals
- Condition : Trametinib (20.0 nM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 1,209 | Full Dataset Size: 19,114
- Experimental Setup : Drug Exposure | Duration: 38 Days
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of the hit gene results in increased proliferation in the presence of high-dose trametinib
Score Distribution
< 14.61144158
> 1.314050928