CRISPR Screen Dataset
Schubert OT (2022) - 6-PMID36326816
Title: Genome-wide base editor screen identifies regulators of protein abundance in yeast.
Screen Details:
- Screen Rationale : Increased/decreased protein abundance (RPL9A (YGL147C))
- Cell Type : Saccharomyces Cerevisiae [NCBI Taxid 4932]
- Cell Line : BY4741 [EFO:0000098]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Yeast Editing Library (Kruglyak, 2022) | Type: Cytosine Base Editing-Mediated Gene Perturbation | Format: Pool | Enzyme: BE3 | Methodology: Perturbation
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 286 | Full Dataset Size: 4,493
- Experimental Setup : Timecourse | Duration: 9 Hours
- Significance : Authors have indicated that results with Score.2 (q-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes whose perturbation by a cytosine base editor resulted in modified abundance of a GFP tagged reporter protein (RPL9A (YGL147C))
- Authors used FACS to isolate cell populations which very high or very low GFP.
- Genes with a significant negative logfold change show high abundance in low GFP populations compared to high GFP populations suggesting perturbation of the gene results in decreased abundance of the tagged gene.
- Genes with a signigicant positive logfold change show high abundance in high GFP populations compared to low GFP populations suggesting perturbation of the hit gene results in increased abundance of the tagged gene.
Score Distribution
< 1.33
> -2.45