CRISPR Screen Dataset
Hou J (2023) - 2-CUSTOM00000021
Title: Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infection
Screen Details:
- Screen Rationale : Decreased resistance to virus
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A549-AC (hACE2 and Cas9)
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (isolate USA-WA1/2020) (5 MOI)
- Library : Liu Human CRISPR Knockout Library (CRISPRn H1 H2, H1/H2) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 84 | Full Dataset Size: 84
- Experimental Setup : Virus Exposure | Duration: 2 Days | MOI: ~ 0.15
- Significance : Authors have indicated that results with Score.1 (Log2FC) <= 0.5 AND Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide loss-of-function CRISPR screen was performed to identify anti-viral host factors depleted after SARS-CoV-2 infection (LOF-depleted data set). The preprint supplementary files only included datasets with gene hits.
Score Distribution
< -0.50536
> -1.5607