CRISPR Screen Dataset
Ortega-Gonzalez P (2022) - 1-PMID35263388
Title: Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Microglial Cell Line [BTO:0003349]
- Cell Line : BV-2 [BTO:0003350, CELLOSAURUS:CVCL_0182]
- Phenotype : Response To Virus
- Condition : Virus: Reovirus strains T1L or T3D (100.0 PFU/cell)
- Library : Asagio (mouse) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 873 | Full Dataset Size: 20,077
- Experimental Setup : Virus Exposure | Duration: 9 Days | MOI: = 0.2
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of the hit gene results in greater resistance to virus
Score Distribution
< 15.43897608
> 2.000281881