CRISPR Screen Dataset
Luu AP (2021) - 1-PMID34834920
Title: A CRISPR Activation Screen Identifies an Atypical Rho GTPase That Enhances Zika Viral Entry.
Screen Details:
- Screen Rationale : Increased sensitivity to virus
- Cell Type : Fibroblast Cell Line [BTO:0000453]
- Cell Line : STAT1−/− fibroblasts
- Phenotype : Response To Virus
- Condition : Virus: Zika virus (0.5 MOI) [NCBI Taxid 64320]
- Library : Human SAM library in lenti-puro backbone [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 2 | Full Dataset Size: 23,728
- Experimental Setup : Virus Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.001 AND Score.3 (FDR) < 0.052 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Negatively selected sgRNAs in surviving ZIKV-infected cell populations target candidate genes hypothesized to activate host proviral factors.
Score Distribution
< 0.99995
> 0.00000143