CRISPR Screen Dataset
Kamber RA (2021) - 5-PMID34497417
Title: Inter-cellular CRISPR screens reveal regulators of cancer cell phagocytosis.
Screen Details:
- Screen Rationale : Regulators of selective antibody-dependent cellular phagocytosis of APMAP-KO cells
- Cell Type : Macrophage [BTO:0002278]
- Cell Line : J774 [BTO:0002279, CELLOSAURUS:CVCL_4692]
- Phenotype : Phagocytosis
- Condition : Exposure to APMAP-KO cancer cells vs. Control-KO cancer cells
- Library : Bassik Mouse CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 24 | Full Dataset Size: 22,894
- Experimental Setup : Other | Duration: 72 Hours
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes with a negative castle effect results in decreased phagocytosis of APMAP-KO, but not control-KO cancer cells
- KO of hit genes with a positive castle effect results in increased phagocytosis of APMAP-KO, but not control-KO cancer cells
Score Distribution
< 55.3
> 0