CRISPR Screen Dataset
Kamber RA (2021) - 2-PMID34497417
Title: Inter-cellular CRISPR screens reveal regulators of cancer cell phagocytosis.
Screen Details:
- Screen Rationale : Regulators of cancer response to antibody-dependent cellular phagocytosis (ADCP)
- Cell Type : Burkitt Lymphoma Cell Line [BTO:0000889]
- Cell Line : Ramos [BTO:0003079, CELLOSAURUS:CVCL_0597, DEPMAP:ACH-001636]
- Phenotype : Cell Proliferation
- Condition : J774 macrophages; anti-CD20/anti-CD47
- Library : CRISPRa-v2 (activation) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: dCas9–VPR | Methodology: Activation
- Analysis Method : CasTLE | Number of Hits: 343 | Full Dataset Size: 18,913
- Experimental Setup : Other | Duration: 8 Days | MOI: = 0.2
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a negative Castle effect are pro-phagocytic, genes whose overexpression decreases cancer cell survival upon incubation with macrophages and anti-CD20 and anti-CD47 antibodies
- Genes with a positive Castle effect are anti-phagocytic, genes whose overexpression increases cancer cell survival upon incubation with macrophages and anti-CD20 and anti-CD47 antibodies
Score Distribution
< 696
> 0