CRISPR Screen Dataset
Liu H (2022) - 1-PMID35411049
Title: Ubiquitin-like protein 3 (UBL3) is required for MARCH ubiquitination of major histocompatibility complex class II and CD86.
Screen Details:
- Screen Rationale : Regulators of March1 mediated ubiquitination of MHC-II
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 12 | Full Dataset Size: 19,050
- Experimental Setup : Timecourse | Duration: 10 Days | MOI: ~ 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Hit cells are enriched in cells sorted for high MHC-II expression, suggesting their KO resulted in decreased ubiquitination by MARCH1
Score Distribution
< 6.04
> 2.19