CRISPR Screen Dataset
Kamber RA (2021) - 1-PMID34497417
Title: Inter-cellular CRISPR screens reveal regulators of cancer cell phagocytosis.
Screen Details:
- Screen Rationale : Regulators of cancer response to antibody-dependent cellular phagocytosis (ADCP)
- Cell Type : Burkitt Lymphoma Cell Line [BTO:0000889]
- Cell Line : Ramos [BTO:0003079, CELLOSAURUS:CVCL_0597, DEPMAP:ACH-001636]
- Phenotype : Cell Proliferation
- Condition : J774 macrophages; anti-CD20
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 90 | Full Dataset Size: 20,531
- Experimental Setup : Other | Duration: 8 Days | MOI: = 0.2
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased sensitivity of cancer cells to antibody-dependent killing by macrophages (negative Castle effect) or decreased sensitivity to antibody-dependent killing by macrophages (positive Castle effect)
Score Distribution
< 338
> 0