CRISPR Screen Dataset
Wang Y (2022) - 1-PMID35482054
Title: Rapid Intraspecies Evolution of Fitness Effects of Yeast Genes.
Screen Details:
- Screen Rationale : Essential genes in a BY4741 Saccharomyces cerevisiae strain
- Cell Type : Saccharomyces Cerevisiae [NCBI Taxid 4932]
- Cell Line : BY4741 [EFO:0000098]
- Phenotype : Cell Proliferation
- Library : S. cerevisiae CRISPRn library (Liu, 2022) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,299 | Full Dataset Size: 5,630
- Experimental Setup : Timecourse | Duration: 5 Days
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 0.1 AND Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- sgRNAs were designed using the Saccharomyces_cerevisiae_S288c reference genome but experiments were performed in strains of interest in order to compare essentiality across strains.
- Experiment performed in BY4741 strain
Score Distribution
< 1
> 0.00000534