CRISPR Screen Dataset
Rebendenne A (2022) - 3-PMID35879413
Title: Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
- Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 ( isolate BetaCoV/France/ IDF0372/2020) (0.005 MOI)
- Library : Human CRISPR Activation Pooled Library (Calabrese) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: dCAS-VP64_Blast (Zhu, 2021) | Methodology: Activation
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 119 | Full Dataset Size: 20,000
- Experimental Setup : Virus Exposure | Duration: 11 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) > 1.96 OR Score.1 (Z-score) < -1.96 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPRa activation screen resulting in overexpression of genes was carried out in Calu-3 cells exposed to the SARS-CoV-2 isolate BetaCoV/France/IDF0372/2020 . In contrast to CRISPRn knockout screens, CRISPRa activation screens identify genes that when overexpressed result in enriched antiviral gene hits (positive scores) and depleted proviral gene hits (negative scores).
Score Distribution
< 2.971123671
> -5.882190564