CRISPR Screen Dataset
Rebendenne A (2022) - 2-PMID35879413
Title: Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
- Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 ( isolate BetaCoV/France/ IDF0372/2020) (0.005 MOI)
- Library : Gattinara (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 102 | Full Dataset Size: 20,513
- Experimental Setup : Virus Exposure | Duration: 30 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) > 1.96 OR Score.1 (Z-score) < -1.96 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR knockout screen was carried out in Calu-3 cells exposed to the SARS-CoV-2 isolate BetaCoV/France/IDF0372/2020 to identify enriched proviral gene hits (positive scores). No antiviral gene hits (negative scores) were identified in this screen.
Score Distribution
< 8.050874068
> -1.588184284