CRISPR Screen Dataset
Freeman AJ (2019) - 1-PMID31509742
Title: Natural Killer Cells Suppress T Cell-Associated Tumor Immune Evasion.
Screen Details:
- Screen Rationale : Tumor cell response to NK cells
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : B16-F10 [BTO:0002014, CELLOSAURUS:CVCL_0159]
- Phenotype : Cell Proliferation
- Condition : NK (activated natural killer) cells (1 Ratio of effector cells/target cells)
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,048 | Full Dataset Size: 20,229
- Experimental Setup : NK cell exposure | Duration: 3 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for tumor-specific genes that are associated with NK cell sensitivity
- Hit genes increased sensitivity to NK cell exposure upon KO
Score Distribution
< 0.99995
> 0.000000601