CRISPR Screen Dataset
Schmidt R (2022) - 2-PMID35113687
Title: CRISPR activation and interference screens decode stimulation responses in primary human T cells.
Screen Details:
- Screen Rationale : Regulation of cytokine production
- Cell Type : T Cell [CL:0000084]
- Cell Line : Primary T-cells
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Interleukin-2 (IL-2) (500 IU/ml) [Uniprot]
- Library : Human CRISPR Activation Pooled Library (Calabrese) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: dCas9-VP64 | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 572 | Full Dataset Size: 18,930
- Experimental Setup : Cytokine exposure | Duration: 8 Days | MOI: ~ 0.5
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Genes with a positive z-score are enriched in an IFN-gamma high population upon activation, suggesting the gene activity increases cytokine production
- Genes with a negative z-score are depleted in an IFN-gamma high population upon activation, suggesting the gene activity inhibits cytokine production
Score Distribution
< 13.11934358
> -10.73301146