CRISPR Screen Dataset
Grodzki M (2022) - 12-PMID35086559
Title: Genome-scale CRISPR screens identify host factors that promote human coronavirus infection.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T/ACE2 [CELLOSAURUS:CVCL_C9BE]
- Phenotype : Response To Virus
- Condition : Virus: HCoV-OC43 (Human coronavirus OC43) (0.1 MOI) [NCBI Taxid 31631]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 88 | Full Dataset Size: 19,115
- Experimental Setup : Virus Exposure | Duration: 32 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (FDR) < 0.25 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR screen was carried out in HEK293T-hACE2 cells to identify host factors required for HCoV-OC43 infection.
- (File 8 HEK-OC43_Reinfect-MOI-0.1-32d: MAGeCK HEK293T-Ace2 results for initial HCoV-OC43 infection at MOI 0.1 for an extra 9 days, 32 days total)
Score Distribution
< 1
> 0.000309