CRISPR Screen Dataset
Potting C (2018) - 2-PMID29269392
Title: Genome-wide CRISPR screen for PARKIN regulators reveals transcriptional repression as a determinant of mitophagy.
Screen Details:
- Screen Rationale : Negative regulators of parkin abundance (HEK293 cells)
- Screen Type : Phenotype Screen | Format: Pool
- Cell Line : HEK-293 cell | Type: Embryonic Kidney Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Protein/peptide Accumulation
- Experimental Setup : Timecourse | Duration: 15 Days | MOI: = 0.5
- Library : CRISPRn (Helliwell, 2018) | Type: CRISPRn | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 53 | Full Dataset Size: 18,703
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- GFP-low populations are enriched for cells expressing sgRNAs that negatively regulate GFP-PARKIN
Score Distribution
< 49.91241175
> -0.268895895