CRISPR Screen Dataset
Grodzki M (2022) - 2-PMID35086559
Title: Genome-scale CRISPR screens identify host factors that promote human coronavirus infection.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : African Green Monkey Kidney Cell Line
- Cell Line : Vero-E6|VERO C1008 [BTO:0004755, COVOC:0020002, CELLOSAURUS:CVCL_0574]
- Phenotype : Response To Virus
- Condition : Virus: HCoV-OC43 (Human coronavirus OC43) (0.01 MOI) [NCBI Taxid 31631]
- Library : African green monkey CRISPRn (Norris, 2022) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 13 | Full Dataset Size: 19,415
- Experimental Setup : Virus Exposure | Duration: 28 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (FDR) < 0.25 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR screen was carried out in Vero E6 cells to identify African green monkey host factors required for human coronavirus OC43 (HCoV-OC43) infection. This screen contains the results from the initial infection with HCoV-OC43 at an MOI = 0.01 for 28 days.
Score Distribution
< 0.999982
> 0.000707