CRISPR Screen Dataset
Potting C (2018) - 1-PMID29269392
Title: Genome-wide CRISPR screen for PARKIN regulators reveals transcriptional repression as a determinant of mitophagy.
Screen Details:
- Screen Rationale : Positive regulators of parkin abundance
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : CRISPRn (Helliwell, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 60 | Full Dataset Size: 18,703
- Experimental Setup : Timecourse | Duration: 15 Days | MOI: = 0.5
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- GFP-high populations are enriched for cells expressing sgRNAs that positively regulate GFP-PARKIN
Score Distribution
< 0.422235445
> -38.36477865