CRISPR Screen Dataset

Kratzel A (2021) - 3-PMID34962926

H. sapiens

Title: A genome-wide CRISPR screen identifies interactors of the autophagy pathway as conserved coronavirus targets.

Screen Rationale : Increased resistance to virus
Cell Type : Hepatoma Cell Line [BTO:0000578]
Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
Phenotype : Response To Virus
Condition : Virus: HCoV-229E (Human coronavirus 229E) (0.1 MOI) [NCBI Taxid 11137]
Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 926 | Full Dataset Size: 21,668
Experimental Setup : Virus Exposure | Duration: 14 Days | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (p-Value) <= 0.05 AND Score.2 (Log2FC) >= 2.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A genome-scale loss-of-function CRISPR screen was performed to identify host dependency factors (HDFs) essential for HCoV-229E coronavirus infection (alpha median method for calculating LFCs).

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