CRISPR Screen Dataset

Vichas A (2021) - 3-PMID34373451

H. sapiens

Title: Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.

Screen Rationale : Cell-essential genes and growth restrictive genes in a KRASG12V mutant background
Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
Cell Line : PC-9 [BTO:0005518, CELLOSAURUS:CVCL_B260]
Phenotype : Cell Proliferation
Condition : Mutation: KRAS (ETG3845) G12V
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 1,637 | Full Dataset Size: 19,363
Experimental Setup : Timecourse | Duration: 12 Doublings | MOI: < 0.3
Significance : Authors have indicated that results with Score.1 (CRISPR Score (CS)) < -0.5 AND Score.2 (Log10 (p-value)) > 1.3 OR Score.1 (CRISPR Score (CS)) > 0.5 AND Score.2 (Log10 (p-value)) > 1.3 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Genes with a significant negative CRISPR Score confer decreased growth in an KRAS mutant background upon KO (essential), genes with a significant positive CRISPR score confer increased growth in an KRAS mutant background upon KO (growth restrictive)

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