CRISPR Screen Dataset
Shen L (2021) - 1-PMID34907164
Title: CRISPR/Cas9 genome-wide screening identifies LUC7L2 that promotes radioresistance via autophagy in nasopharyngeal carcinoma cells.
Screen Details:
- Screen Rationale : Increased sensitivity to radiation
- Cell Type : Nasopharyngeal Carcinoma Cell Line [BTO:0003960]
- Cell Line : CNE2-IR [CELLOSAURUS:CVCL_C9B8]
- Phenotype : Response To Radiation
- Condition : Ionizing Radiation (IR) (24 Gy)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Differential CRISPR score | Number of Hits: 10 | Full Dataset Size: 19,114
- Experimental Setup : Radiation Exposure | Duration: 2 Months
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Cells were exposed to 6 Gy/cycle for up to 4 cycles for a possible cumulative dosage of 24 Gy
- CNE-2(IR) cells are a subset of CNE-2 (nasopharyngeal carcinoma cell line) that are resistant to radiation
Score Distribution
< 1
> 1.54e-16