CRISPR Screen Dataset
Gulbranson DR (2017) - 1-PMID28894007
Title: RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.
Screen Details:
- Screen Rationale : Exocytosis (HeLa cells exposed to insulin)
- Screen Type : Phenotype Screen | Format: Pool
- Cell Line : HeLa | Type: Cervical Adenocarcinoma Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Protein Transport
- Experimental Setup : Ligand Exposure | Duration: 3 Hours | Condition Name: Insulin (100 nM) | MOI: ~ 0.4
- Library : GECKO v2 | Type: CRISPRn | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 329 | Full Dataset Size: 18,737
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened cells for exocytosis defects. Hela cells expressing tagged GLUT4 were exposed to insulin and then FACs sorted to identify cells with reduced surface expression of Glut4.
Score Distribution
< 0.99997
> 0.000000000015