CRISPR Screen Dataset
Gulbranson DR (2017) - 1-PMID28894007
Title: RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.
Screen Details:
- Screen Rationale : Exocytosis
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Protein Transport [APO:0000129]
- Condition : Insulin (100 nM) [Uniprot]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 329 | Full Dataset Size: 18,737
- Experimental Setup : Ligand Exposure | Duration: 3 Hours | MOI: ~ 0.4
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened cells for exocytosis defects. Hela cells expressing tagged GLUT4 were exposed to insulin and then FACs sorted to identify cells with reduced surface expression of Glut4.
Score Distribution
< 0.99997
> 0.000000000015