CRISPR Screen Dataset

Riba A (2017) - 6-PMID28215525

H. sapiens

Title: Explicit Modeling of siRNA-Dependent On- and Off-Target Repression Improves the Interpretation of Screening Results.

Screen Rationale : Negative regulators of NFkB signaling pathway
Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
Cell Line : HEK-293 [BTO:0000007, CELLOSAURUS:CVCL_0045]
Phenotype : Regulation Of Signal Transduction Phenotype [CMPO:0000040]
Condition : Interleukin-1 β (0.3 ng/mL) [Uniprot]
Library : CRISPRn (Zavolan, 2017) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : RSA | Number of Hits: 113 | Full Dataset Size: 18,671
Experimental Setup : Ligand Exposure | Duration: 8 Days | MOI: = 0.5
Significance : Authors have indicated that results with Score.1 (Log10 (p-value)) < -4.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Cells with high reporter expression should be those in which the sgRNA targeted an repressor of the NFkB pathway.

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