CRISPR Screen Dataset
Emmer BT (2021) - 2-PMID33513160
Title: Genome-scale CRISPR screening for modifiers of cellular LDL uptake.
Screen Details:
- Screen Rationale : Regulation of LDL uptake
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : lipoprotein-depleted culture conditions
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 47 | Full Dataset Size: 21,663
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 OR Score.4 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit genes results in reduced LDL uptake in lipoprotein-depleted culture conditions, except for SQLE which was the sole gene in this screen whose KO resulted in increased LDL uptake
Score Distribution
< 1
> 0.0000000000588