Screen Summary
Riba A (2017) - 5-PMID28215525
Title: Explicit Modeling of siRNA-Dependent On- and Off-Target Repression Improves the Interpretation of Screening Results.
Screen Details:
- Screen Type : Phenotype Screen | Format: Pool
- Cell Line : HEK-293 cell | Type: Embryonic Kidney Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Regulation Of Signal Transduction Phenotype
- Experimental Setup : Ligand Exposure | Duration: 8 Days | Condition Name: Interleukin-1 β (0.3 ng/mL) | MOI: = 0.5
- Library : CRISPRn (Zavolan, 2017) | Type: CRISPRn | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 371 | Full Dataset Size: 18,671
- Significance : Authors have indicated that results with Score.1 (Log10 (p-value)) < -4 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Cells with low reporter expression should be those in which the sgRNA targeted an activator of the NFkB pathway.
Score Distribution
< -0.000445548
> -18.82122872