CRISPR Screen Dataset
Riba A (2017) - 5-PMID28215525
Title: Explicit Modeling of siRNA-Dependent On- and Off-Target Repression Improves the Interpretation of Screening Results.
Screen Details:
- Screen Rationale : Positive regulators of NFkB signaling pathway
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK-293 [BTO:0000007]
- Phenotype : Regulation Of Signal Transduction Phenotype [CMPO:0000040]
- Condition : Interleukin-1 β (0.3 ng/mL) [Uniprot]
- Library : CRISPRn (Zavolan, 2017) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 371 | Full Dataset Size: 18,671
- Experimental Setup : Ligand Exposure | Duration: 8 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.1 (Log10 (p-value)) < -4.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Cells with low reporter expression should be those in which the sgRNA targeted an activator of the NFkB pathway.
Score Distribution
< -0.000445548
> -18.82122872