CRISPR Screen Dataset
Gaudet RG (2021) - 1-PMID34437126
Title: A human apolipoprotein L with detergent-like activity kills intracellular pathogens.
Screen Details:
- Screen Rationale : Increased susceptibility to bacterial infection
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Response To Bacteria
- Condition : Salmonella enterica serovar Typhimurium and IFNgamma (500 U/ml) (20 MOI)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 2 | Full Dataset Size: 18,974
- Experimental Setup : Bacteria Exposure | Duration: 48 Hours
- Significance : Authors have indicated that results with Score.1 (p-Value) < 0.001 AND Score.2 (mean fold change) > 4.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene resulted in increased Salmonella enterica serovar Typhimurium (Stm) infection, even when IFNgamma was stimulated, suggesting that the gene normally plays a role in IFNgamma mediated bacterial resistance
Score Distribution
< 0.99997
> 0.000081496