CRISPR Screen Dataset

Akinci E (2022) - 2-CUSTOM00000017

H. sapiens

Title: Elucidation of remdesivir cytotoxicity pathways through genome-wide CRISPR-Cas9 screening and transcriptomics

Screen Rationale : Increased/decreased resistance to drug
Cell Type : Colonic Adenocarcinoma Cell Line [BTO:0001913]
Cell Line : HT-29 [BTO:0000182, CELLOSAURUS:CVCL_0320]
Phenotype : Response To Chemicals
Condition : Hydroxychloroquine (30.0 µM) [ChEBI]
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MAGeCK-MLE | Number of Hits: 1,330 | Full Dataset Size: 19,114
Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: ~ 0.5
Significance : Authors have indicated that results with Score.1 (Beta Score) < 0.0 AND Score.2 (FDR) < 0.01 OR Score.1 (Beta Score) > 0.0 AND Score.2 (FDR) < 0.01 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A CRISPR screen was carried out to identify affected genes in the hydroxychloroquine (HDC) treated cells. The top gene hits with an FDR < 0.01 are noted. Negative beta scores indicate depleted genes and positive beta scores indicate enriched genes.

< 1.1339

> -1.6266