CRISPR Screen Dataset
Schraivogel D (2022) - 2-PMID35050652
Title: High-speed fluorescence image-enabled cell sorting.
Screen Details:
- Screen Rationale : Increased/decreased protein localization
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa RelA-mNeonGreen [CELLOSAURUS:CVCL_C9AQ]
- Phenotype : Protein/peptide Distribution [APO:0000209]
- Condition : IFN-β (Interferon beta) (75 ng/µL)
- Library : CRISPRn (Steinmetz, 2022) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAUDE | Number of Hits: 169 | Full Dataset Size: 18,408
- Experimental Setup : Cytokine exposure | Duration: 60 Minutes
- Significance : Authors have indicated that results with Score.1 (Z-score) < 0.0 AND Score.2 (FDR) < 0.01 OR Score.1 (Z-score) > 0.0 AND Score.2 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR screen was combined with high-speed image-enabled cell sorting (ICS) to quantify the nuclear translocation of fluorescently tagged RelA upon NF-kB pathway activation by TFNalpha. Positive regulators of RelA nuclear translocation are enriched (negative Z-score) and negative regulators are depleted (positive Z-score) (FDR < 1%).
Score Distribution
< 9.916538125
> -46.59000838