CRISPR Screen Dataset
Estoppey D (2021) - 4-PMID34805786
Title: Genome-wide CRISPR-Cas9 screens identify mechanisms of BET bromodomain inhibitor sensitivity.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Colorectal Cancer Cell Line [BTO:0001616]
- Cell Line : HCT 116 [BTO:0001109, CELLOSAURUS:CVCL_0291]
- Phenotype : Response To Chemicals
- Condition : JQ1 (0.2 µM) [ChEBI]
- Library : CRISPRn (Kirkland, 2021) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 301 | Full Dataset Size: 18,360
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: ~ 0.5
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of hit genes results in increased resistance to JQ1
- Authors used a threshold of RSA.Up < -2 for significance
Score Distribution
< -2.000786995
> -5.717876282