CRISPR Screen Dataset
Condon KJ (2021) - 1-PMID33483422
Title: Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction.
Screen Details:
- Screen Rationale : mTORC1 activation
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 173 | Full Dataset Size: 20,044
- Experimental Setup : Timecourse | Duration: 10 Days
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- To identify genes that contribute to mTORC1 activation, the authors used a fluorescence-activated cell sorting (FACS)-based CRISPR-Cas9 screening strategy that uses the phosphorylation of rpS6, a well-established marker of mTORC1 activity, as a readout
- Hit genes are positive regulators of mTORC1 activity
- Screen performed in AMPK double knock-out cells
Score Distribution
< 1
> 7.5823e-16