CRISPR Screen Dataset
Danziger O (2022) - 2-CUSTOM00000014
Title: Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor.
Screen Details:
- Screen Rationale : Increased/decreased proliferation
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A549-deltaSTAT1-SunTag [CELLOSAURUS:CVCL_C9AU]
- Phenotype : Cell Proliferation
- Condition : Doxycycline (5.0 µg/mL) [ChEBI]
- Library : Modified Calabrese Library (414 interferon-stimulated genes) (Danziger, 2021) | Type: CRISPRa | Format: Pool | Enzyme: dCas9-SunTag-P2A-HygR | Methodology: Activation
- Analysis Method : MAGeCK-MLE | Number of Hits: 79 | Full Dataset Size: 414
- Experimental Setup : Drug Exposure | Duration: 72 Hours | MOI: = 0.1
- Significance : Authors have indicated that results with Score.1 (Beta Score) < 0.0 AND Score.2 (p-Value) < 0.018 OR Score.1 (Beta Score) > 0.0 AND Score.2 (p-Value) < 0.018 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A doxycline-inducible CRISPRa activation screen focused on 414 interferon-stimulated genes was performed to identify antiproliferative and proproliferative (proapoptotic) genes in STAT1 knockout A549-deltaSTAT1-SunTag lung cancer cells. The A549-deltaSTAT1-SunTag cell line has STAT1 knocked out and therefore has a defective interferon (IFN) response.
Score Distribution
< 0.6668141433
> -1.12919827