CRISPR Screen Dataset
Goh CJH (2021) - 1-PMID33604667
Title: Identification of pathways modulating vemurafenib resistance in melanoma cells via a genome-wide CRISPR/Cas9 screen.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : A-375 [BTO:0002806, CELLOSAURUS:CVCL_0132]
- Phenotype : Response To Chemicals
- Condition : Vemurafenib (2.0 µM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CRISPRanalyzer | Number of Hits: 33 | Full Dataset Size: 18,960
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes confers resistance to vemurafenib
Score Distribution
< 1
> 0.0004