CRISPR Screen Dataset
Blondel CJ (2016) - 4-PMID27453484
Title: CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity.
Screen Details:
- Screen Rationale : Increased resistance to toxin
- Cell Type : Colonic Adenocarcinoma Cell Line [BTO:0001913]
- Cell Line : HT-29 [BTO:0000182, CELLOSAURUS:CVCL_0320]
- Phenotype : Response To Toxin
- Condition : V. parahaemolyticus with active T3SS2
- Library : Avana | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 1,046 | Full Dataset Size: 18,675
- Experimental Setup : Toxin Exposure | Duration: 3 Hours | MOI: = 1.0
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Phenotypic readout: cell proliferation
- HT-29 cells were exposed to the gram-negative marine bacterium V. parahaemolyticus which usually expresses two type III secretion systems: T3SS1 and T3SS2. In this screen T3SS1 has been inactivated and only T3SS2 is functional. Cells were screened for gene knock-outs that conferred resistance to T3SS2 induced toxicity.
- This screen is one of two biological replicates from this paper (screen 3 and 4 are replicates)
Score Distribution
< 13.0847401
> 0.865660011