CRISPR Screen Dataset

Blondel CJ (2016) - 4-PMID27453484

Title: CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity.

Screen Details:

  • Phenotypic readout: cell proliferation
  • HT-29 cells were exposed to the gram-negative marine bacterium V. parahaemolyticus which usually expresses two type III secretion systems: T3SS1 and T3SS2. In this screen T3SS1 has been inactivated and only T3SS2 is functional. Cells were screened for gene knock-outs that conferred resistance to T3SS2 induced toxicity.
  • This screen is one of two biological replicates from this paper (screen 3 and 4 are replicates)

Score Distribution

< 13.0847401
> 0.865660011

CRISPR Screen Results (1046 hits)