CRISPR Screen Dataset
Biering SB (2022) - 3-PMID35879412
Title: Genome-wide bidirectional CRISPR screens identify mucins as host factors modulating SARS-CoV-2 infection.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
- Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (isolate USA-WA1/2020) (0.05 MOI)
- Library : Human CRISPR Activation Pooled Library (Calabrese) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: dCas9-VP64 + PP7-P65-HSF1 | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 91 | Full Dataset Size: 18,884
- Experimental Setup : Virus Exposure | Duration: 5 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-scale gain-of-function CRISPR screen was performed to identify antiviral host factors modulating SARS-CoV-2 infection (GOF-enriched data set).
Score Distribution
< 1
> 1.09e-26