CRISPR Screen Dataset
Goodspeed A (2019) - 1-PMID30414698
Title: A Whole-genome CRISPR Screen Identifies a Role of MSH2 in Cisplatin-mediated Cell Death in Muscle-invasive Bladder Cancer.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Urinary Bladder Cancer Cell Line [BTO:0000792]
- Cell Line : MGH-U4 [BTO:0006288, CELLOSAURUS:CVCL_9828]
- Phenotype : Response To Chemicals
- Condition : Cisplatin (3 µM) [ChemSpider]
- Library : GECKO | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DESeq2 | Number of Hits: 7,367 | Full Dataset Size: 16,564
- Experimental Setup : Drug Exposure | Duration: 30 Hours | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes that conferred resistance to cisplatin
Score Distribution
< 1
> 1.1e-37