CRISPR Screen Dataset
Hoshino A (2019) - 4-PMID31618756
Title: The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.
Screen Details:
- Screen Rationale : Regulators of mitophagy
- Cell Type : Myoblast Cell Line [BTO:0000256]
- Cell Line : C2C12 [BTO:0000165, CELLOSAURUS:CVCL_0188]
- Phenotype : Protein/peptide Distribution [APO:0000209]
- Condition : CCCP (13.0 µM) [ChEBI]
- Library : GECKO v2 (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Delta Z scores | Number of Hits: 2,684 | Full Dataset Size: 19,983
- Experimental Setup : Drug Exposure | Duration: 10 Days | MOI: ~ 0.5
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- The authors used altered fluorescence of matrix-targeted mKeima from 440 to 586 as it encounters the low pH environment of the lysosome as a marker for mitophagy
Score Distribution
< 2.89840675
> -1.820277513